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Dityrosine antibody (Atto 594)

DT WB, ELISA, ICC, IF, FACS Host: Mouse Monoclonal 10A6 Atto 594
Catalog No. ABIN5067473
  • Target See all Dityrosine (DT) products
    Dityrosine (DT)
    Host
    • 11
    Mouse
    Clonality
    • 11
    Monoclonal
    Conjugate
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Dityrosine antibody is conjugated to Atto 594
    Application
    • 11
    • 10
    • 9
    • 9
    • 9
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
    Specificity
    Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
    Purification
    Protein G Purified
    Immunogen
    Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
    Clone
    10A6
    Isotype
    IgG1
  • Application Notes
    • WB (1:1000)
    • ICC/IF (1:50)
    • FACS (1:50)
    • FCM (1:50)
    • ELISA (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Comment

    A 1:1000 dilution of ABIN5067473 was sufficient for detection of dityrosine in 1 μg of Dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    Dityrosine (DT)
    Alternative Name
    Dityrosine (DT Products)
    Target Type
    Dipeptide
    Background
    ROS such as hydrogen peroxide (H2O2), superoxide, and hydroxyl radicals can react with both the backbone and the side chains of proteins, leading to backbone cleavage and side-chain modifications, respectively. Peroxidases, UV radiation, and hydroxyl radicals catalyze the formation of tyrosyl radicals which then react to form cross-links between proteins (1). This produces dityrosine, a metabolically stable biomarker of protein oxidation (2).
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