Dityrosine antibody (PE)
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- Target See all Dityrosine (DT) products
- Dityrosine (DT)
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Dityrosine antibody is conjugated to PE
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Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Specificity
- Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
- Purification
- Protein G Purified
- Immunogen
- Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
- Clone
- 10A6
- Isotype
- IgG1
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- Application Notes
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- WB (1:1000)
- ICC/IF (1:50)
- FACS (1:50)
- FCM (1:50)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
- Comment
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A 1:1000 dilution of ABIN5067485 was sufficient for detection of dityrosine in 1 μg of Dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- Dityrosine (DT)
- Alternative Name
- Dityrosine (DT Products)
- Target Type
- Dipeptide
- Background
- ROS such as hydrogen peroxide (H2O2), superoxide, and hydroxyl radicals can react with both the backbone and the side chains of proteins, leading to backbone cleavage and side-chain modifications, respectively. Peroxidases, UV radiation, and hydroxyl radicals catalyze the formation of tyrosyl radicals which then react to form cross-links between proteins (1). This produces dityrosine, a metabolically stable biomarker of protein oxidation (2).
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