SETD7 antibody (AA 1-366)

Catalog No. ABIN532913

Product Details anti-SETD7 Antibody

Target: SETD7 (SET Domain Containing (Lysine Methyltransferase) 7 (SETD7))

Binding Specificity: AA 1-366

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SETD7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purpose: Mouse monoclonal antibody raised against partial recombinant SETD7.

Cross-Reactivity: Human

Immunogen: Recombinant protein corresponding to amino acids 1-366 of human SETD7.

Clone: S4E5

Isotype: IgG2b

Application Details

Application Notes: Western Blot (1:250-1:2000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS, pH 7.4 (10 % glycerol, 0.02 % sodium azide).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-SETD7 antibody (ABIN532913)

Hotz, Peters: "Protein demethylation required for DNA methylation." in: Nature genetics, Vol. 41, Issue 1, pp. 10-1, (2008) (PubMed).

Kwon, Chang, Kwak, Lee, Joachimiak, Kim, Lee, Cho: "Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet." in: The EMBO journal, Vol. 22, Issue 2, pp. 292-303, (2003) (PubMed).

Xiao, Jing, Wilson, Walker, Vasisht, Kelly, Howell, Taylor, Blackburn, Gamblin: "Structure and catalytic mechanism of the human histone methyltransferase SET7/9." in: Nature, Vol. 421, Issue 6923, pp. 652-6, (2003) (PubMed).

Nishioka, Chuikov, Sarma, Erdjument-Bromage, Allis, Tempst, Reinberg: "Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation." in: Genes & development, Vol. 16, Issue 4, pp. 479-89, (2002) (PubMed).

Target Details for SETD7

Target: SETD7 (SET Domain Containing (Lysine Methyltransferase) 7 (SETD7))

Alternative Name: SETD7 / SET9 (SETD7 Products)

Synonyms: KMT7 antibody, SET7 antibody, SET7/9 antibody, SET9 antibody, set7 antibody, zgc:101860 antibody, zgc:92330 antibody, 1600028F23Rik antibody, H3K4MT antibody, Set7 antibody, Set7/9 antibody, mKIAA1717 antibody, SET domain containing lysine methyltransferase 7 antibody, SET domain containing (lysine methyltransferase) 7 antibody, SETD7 antibody, setd7 antibody, Setd7 antibody

Gene ID: 80854

NCBI Accession: NM_030648