HSP27 antibody

Catalog No. ABIN532951

Product Details anti-HSP27 Antibody

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purpose: Mouse monoclonal antibody raised against full length recombinant HSPB1.

Cross-Reactivity: Human

Immunogen: Recombinant protein corresponding to full length human HSPB1.

Clone: 2A5

Isotype: IgG1

Application Details

Application Notes: Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS, pH 7.4 (10 % glycerol, 0.02 % sodium azide).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-HSP27 antibody (ABIN532951)

Borrelli, Bernock, Landry, Spitz, Weber, Hickey, Freeman, Corry: "Stress protection by a fluorescent Hsp27 chimera that is independent of nuclear translocation or multimeric dissociation." in: Cell stress & chaperones, Vol. 7, Issue 3, pp. 281-96, (2002) (PubMed).

Butt, Immler, Meyer, Kotlyarov, Laass, Gaestel: "Heat shock protein 27 is a substrate of cGMP-dependent protein kinase in intact human platelets: phosphorylation-induced actin polymerization caused by HSP27 mutants." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7108-13, (2001) (PubMed).

Charette, Lavoie, Lambert, Landry: "Inhibition of Daxx-mediated apoptosis by heat shock protein 27." in: Molecular and cellular biology, Vol. 20, Issue 20, pp. 7602-12, (2000) (PubMed).

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSPB1 / HSP27 (HSPB1 Products)

Synonyms: CMT2F antibody, HMN2B antibody, HS.76067 antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, 27kDa antibody, Hsp27 antibody, cb153 antibody, cb660 antibody, hsp1 antibody, hsp25 antibody, hsp27 antibody, id:ibd2821 antibody, sb:cb660 antibody, zgc:103437 antibody, HSPB1 antibody, cmt2f antibody, hs.76067 antibody, hsp28 antibody, heat shock protein family B (small) member 1 antibody, heat shock protein 1 antibody, heat shock protein, alpha-crystallin-related, 1 antibody, heat shock protein family B (small) member 1 L homeolog antibody, HSPB1 antibody, Hspb1 antibody, hspb1 antibody, hspb1.L antibody

Gene ID: 3315

NCBI Accession: NM_001540

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling