BCAR1 antibody (C-Term)

Catalog No. ABIN533161

Product Details anti-BCAR1 Antibody

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Binding Specificity: C-Term

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCAR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Mouse monoclonal antibody raised against partial recombinant Bcar1.

Specificity: This rat sequence is highly conserved in human, mouse, and chicken p130 Cas.

Cross-Reactivity: Chicken, Human, Mouse, Rat

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: Recombinant protein corresponding to C-terminus of rat Bcar1.

Clone: M144

Isotype: IgG1

Application Details

Application Notes: ELISA (1:2000)
Immunocytochemistry (1:250)
Immunoprecipitation (5-10 µL)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (50 % glycerol, 1 mg/mL BSA, 0.05 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-BCAR1 antibody (ABIN533161)

Shin, Dise, Schneider-Mergener, Ritchie, Kilkenny, Hanks: "Subsets of the major tyrosine phosphorylation sites in Crk-associated substrate (CAS) are sufficient to promote cell migration." in: The Journal of biological chemistry, Vol. 279, Issue 37, pp. 38331-7, (2004) (PubMed).

Hoon Kim, Jeon Choi, Kook, Kim, Keun Song: "Phosphorylation-dependent cleavage of p130cas in apoptotic rat-1 cells." in: Biochemical and biophysical research communications, Vol. 300, Issue 1, pp. 141-8, (2002) (PubMed).

Pellicena, Miller: "Processive phosphorylation of p130Cas by Src depends on SH3-polyproline interactions." in: The Journal of biological chemistry, Vol. 276, Issue 30, pp. 28190-6, (2001) (PubMed).

Target Details for BCAR1

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Alternative Name: BCAR1 / CRKAS (BCAR1 Products)

Synonyms: CAS antibody, CAS1 antibody, CASS1 antibody, CRKAS antibody, P130Cas antibody, AI385681 antibody, Cas antibody, Crkas antibody, P130CAS antibody, MGC68466 antibody, cas antibody, p130cas antibody, BCAR1 antibody, fc17f11 antibody, wu:fc17f11 antibody, zgc:175192 antibody, BCAR1, Cas family scaffolding protein antibody, breast cancer anti-estrogen resistance 1 antibody, breast cancer anti-estrogen resistance 1 L homeolog antibody, breast cancer anti-estrogen resistance protein 1 antibody, BCAR1 antibody, Bcar1 antibody, bcar1.L antibody, bcar1 antibody, LOC100435586 antibody

Gene ID: 25414

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling