HSP27 antibody

Catalog No. ABIN533231

Product Details anti-HSP27 Antibody

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human, Turkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Mouse monoclonal antibody raised against partial native turkey HSPB1.

Immunogen: Native purified HSPB1 from turkey gizzard smooth muscle.

Clone: IAP-9

Isotype: IgG1

Application Details

Application Notes: Immunocytochemistry (1 μg/mL)
Immunohistochemistry (1-2 μg/mL)
Western Blot (0.5-2 μg/mL)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from ascites fluid, 1.2 % sodium acetate, 2 mg BSA, 0.01 mg sodium azide

Storage: -20 °C

Storage Comment: Store at -20°C.
After reconstitution with 1 mL of 1.2% sodium acetate or neutral PBS and concentration will be 100 ug/mL, store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-HSP27 antibody (ABIN533231)

Evgrafov, Mersiyanova, Irobi, Van Den Bosch, Dierick, Leung, Schagina, Verpoorten, Van Impe, Fedotov, Dadali, Auer-Grumbach, Windpassinger, Wagner, Mitrovic, Hilton-Jones, Talbot, Martin, Vasserman et al.: "Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. ..." in: Nature genetics, Vol. 36, Issue 6, pp. 602-6, (2004) (PubMed).

Wyttenbach, Sauvageot, Carmichael, Diaz-Latoud, Arrigo, Rubinsztein: "Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin." in: Human molecular genetics, Vol. 11, Issue 9, pp. 1137-51, (2002) (PubMed).

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSPB1 / HSP27 (HSPB1 Products)

Synonyms: CMT2F antibody, HMN2B antibody, HS.76067 antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, 27kDa antibody, Hsp27 antibody, cb153 antibody, cb660 antibody, hsp1 antibody, hsp25 antibody, hsp27 antibody, id:ibd2821 antibody, sb:cb660 antibody, zgc:103437 antibody, HSPB1 antibody, cmt2f antibody, hs.76067 antibody, hsp28 antibody, heat shock protein family B (small) member 1 antibody, heat shock protein 1 antibody, heat shock protein, alpha-crystallin-related, 1 antibody, heat shock protein family B (small) member 1 L homeolog antibody, HSPB1 antibody, Hspb1 antibody, hspb1 antibody, hspb1.L antibody

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling