Nestin antibody (AA 1464-1614)

Catalog No. ABIN534055

Product Details anti-Nestin Antibody

Target: Nestin (NES)

Binding Specificity: AA 1464-1614

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Mouse monoclonal antibody raised against partial recombinant NES.

Specificity: This antibody is specific to human nestin.

Cross-Reactivity: Human

Immunogen: Recombinant protein corresponding to amino acids 1464-1614 of human NES.

Clone: 10C2

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In buffer containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-Nestin antibody (ABIN534055)

Gangemi, Griffero, Marubbi, Perera, Capra, Malatesta, Ravetti, Zona, Daga, Corte: "SOX2 silencing in glioblastoma tumor-initiating cells causes stop of proliferation and loss of tumorigenicity." in: Stem cells (Dayton, Ohio), Vol. 27, Issue 1, pp. 40-8, (2009) (PubMed).

Messam, Hou, Major: "Coexpression of nestin in neural and glial cells in the developing human CNS defined by a human-specific anti-nestin antibody." in: Experimental neurology, Vol. 161, Issue 2, pp. 585-96, (2000) (PubMed).

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin (NES Products)

Synonyms: paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody

Gene ID: 10763