DIABLO antibody (AA 56-239)

Catalog No. ABIN539482

Product Details anti-DIABLO Antibody

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Binding Specificity: AA 56-239

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIABLO antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Purpose: Rabbit polyclonal antibody raised against partial recombinant DIABLO.

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: Recombinant His fusion protein corresponding to amino acids 56-239 of human DIABLO.

Application Details

Application Notes: Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-DIABLO antibody (ABIN539482)

Wu, Chai, Suber, Wu, Du, Wang, Shi: "Structural basis of IAP recognition by Smac/DIABLO." in: Nature, Vol. 408, Issue 6815, pp. 1008-12, (2001) (PubMed).

Du, Fang, Li, Li, Wang: "Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition." in: Cell, Vol. 102, Issue 1, pp. 33-42, (2000) (PubMed).

Srinivasula, Datta, Fan, Fernandes-Alnemri, Huang, Alnemri: "Molecular determinants of the caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathway." in: The Journal of biological chemistry, Vol. 275, Issue 46, pp. 36152-7, (2000) (PubMed).

Target Details for DIABLO

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Alternative Name: DIABLO / SMAC (DIABLO Products)

Synonyms: smac antibody, smac3 antibody, diablo-s antibody, 0610041G12Rik antibody, 1700006L01Rik antibody, AU040403 antibody, Smac antibody, DFNA64 antibody, SMAC antibody, DBO antibody, XSmac antibody, diablo, IAP-binding mitochondrial protein antibody, diablo IAP-binding mitochondrial protein antibody, diablo, IAP-binding mitochondrial protein L homeolog antibody, diablo antibody, Diablo antibody, DIABLO antibody, diablo.L antibody

Gene ID: 56616

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity