EPAS1 antibody (C-Term)

Catalog No. ABIN540396

Product Details anti-EPAS1 Antibody

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Fish, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPAS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Chromatin Immunoprecipitation (ChIP), Gel Shift (GS)

Purpose: Rabbit polyclonal antibody raised against synthetic peptide of EPAS1.

Specificity: Specific for HIF-2 alpha/EPAS. Does not cross-react with HIF-1 alpha.

Cross-Reactivity: Fish, Human, Mouse, Primate, Rat

Immunogen: A synthetic peptide corresponding to C-terminus of human and mouse EPAS1/Epas1.

Application Details

Application Notes: ChIP (1:10-1:500)
ELISA (1:100-1:2000)
Flow Cytometry
Gel Supershift Assay
Immunocytochemistry (1:100)
Immunofluorescence (1:100)
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sectio

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (0.05 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-EPAS1 antibody (ABIN540396)

Ruf, Moch, Schraml: "PD-L1 expression is regulated by hypoxia inducible factor in clear cell renal cell carcinoma." in: International journal of cancer, Vol. 139, Issue 2, pp. 396-403, (2016) (PubMed).

Wacker, Sachs, Knaup, Wiesener, Weiske, Huber, Akçetin, Behrens: "Key role for activin B in cellular transformation after loss of the von Hippel-Lindau tumor suppressor." in: Molecular and cellular biology, Vol. 29, Issue 7, pp. 1707-18, (2009) (PubMed).

Lee, Qian, Rey, Wei, Liu, Semenza: "Anthracycline chemotherapy inhibits HIF-1 transcriptional activity and tumor-induced mobilization of circulating angiogenic cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 7, pp. 2353-8, (2009) (PubMed).

Walter, Etienne-Selloum, Brasse, Schleiffer, Bekaert, Vanhoutte, Beretz, Schini-Kerth: "Red wine polyphenols prevent acceleration of neovascularization by angiotensin II in the ischemic rat hindlimb." in: The Journal of pharmacology and experimental therapeutics, Vol. 329, Issue 2, pp. 699-707, (2009) (PubMed).

Lomb, Desouza, Franklin, Freeman et al.: "Prolyl hydroxylase inhibitors depend on extracellular glucose and hypoxia-inducible factor (HIF)-2alpha to inhibit cell death caused by nerve growth factor (NGF) deprivation: evidence that HIF-2alpha ..." in: Molecular pharmacology, Vol. 75, Issue 5, pp. 1198-209, (2009) (PubMed).

Sears, Hoppe, Ebrahem, Anand-Apte: "Prolyl hydroxylase inhibition during hyperoxia prevents oxygen-induced retinopathy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 50, pp. 19898-903, (2008) (PubMed).

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: HIF2A / HIF2 alpha (EPAS1 Products)

Synonyms: EPAS1 antibody, ECYT4 antibody, HIF2A antibody, HLF antibody, MOP2 antibody, PASD2 antibody, bHLHe73 antibody, HIF-2alpha antibody, HRF antibody, Hif2a antibody, bhlhe73 antibody, ecyt4 antibody, hif2 antibody, hif2a antibody, hif2alpha antibody, hlf antibody, mop2 antibody, pasd2 antibody, EPAS antibody, EPAS-1 antibody, endothelial PAS domain protein 1 antibody, endothelial PAS domain protein 1 L homeolog antibody, EPAS1 antibody, epas1 antibody, Epas1 antibody, epas1.L antibody

Gene ID: 2034, 13819

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect