Immunocytochemistry (1:50-1:200) Immunofluorescence (1:50-1:200) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In Tris-citrate/phosphate buffer, pH 7.0-8.0 (0.09 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store at 4°C. Do not freeze.
Pattingre, Bauvy, Carpentier, Levade, Levine, Codogno: "Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy." in: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 2719-28, (2009) (PubMed).
Wang, Singh, Massey, Kane, Kaushik, Grant, Xiang, Cuervo, Czaja: "Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus." in: The Journal of biological chemistry, Vol. 283, Issue 8, pp. 4766-77, (2008) (PubMed).
Park, Lee, Kim, Lee, Lee, Kim, Jang, Choi, Kwon, Kim: "A human scFv antibody against TRAIL receptor 2 induces autophagic cell death in both TRAIL-sensitive and TRAIL-resistant cancer cells." in: Cancer research, Vol. 67, Issue 15, pp. 7327-34, (2007) (PubMed).
Massey, Kaushik, Sovak, Kiffin, Cuervo: "Consequences of the selective blockage of chaperone-mediated autophagy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 15, pp. 5805-10, (2006) (PubMed).
Target
Beclin 1 (BECN1)
(Beclin 1, Autophagy Related (BECN1))