ATG5 antibody (AA 1-50)

Catalog No. ABIN540779

Product Details anti-ATG5 Antibody

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Binding Specificity: AA 1-50

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Pig, Xenopus laevis, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit polyclonal antibody raised against synthetic peptide of ATG5.

Specificity: This antibody is selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing a.a. 1-79. The calpain cleaved form of ATG5 is missing a.a. 195-275.

Cross-Reactivity: Cow, Human, Mouse, Pig, Primate, Rat, Xenopus laevis, Zebrafish (Danio rerio)

Immunogen: A synthetic peptide corresponding to amino acids 1-50 of human ATG5.

Application Details

Application Notes: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5 μg/mL)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (30 % glycerol, 0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-ATG5 antibody (ABIN540779)

Wang, Singh, Massey, Kane, Kaushik, Grant, Xiang, Cuervo, Czaja: "Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus." in: The Journal of biological chemistry, Vol. 283, Issue 8, pp. 4766-77, (2008) (PubMed).

Pyo, Nah, Kim, Lee, Heo, Lee, Jung: "Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death." in: Autophagy, Vol. 4, Issue 3, pp. 315-21, (2008) (PubMed).

Cadwell, Liu, Brown, Miyoshi, Loh, Lennerz, Kishi, Kc, Carrero, Hunt, Stone, Brunt, Xavier, Sleckman, Li, Mizushima, Stappenbeck, Virgin: "A key role for autophagy and the autophagy gene Atg16l1 in mouse and human intestinal Paneth cells." in: Nature, Vol. 456, Issue 7219, pp. 259-63, (2008) (PubMed).

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: APG5L / ATG5 (ATG5 Products)

Synonyms: APG5 antibody, APG5-LIKE antibody, APG5L antibody, ASP antibody, hAPG5 antibody, ATG5 antibody, CG1643 antibody, DmAtg5 antibody, Dmel\\CG1643 antibody, atg5 antibody, 2010107M05Rik antibody, 3110067M24Rik antibody, AW319544 antibody, Apg5l antibody, Atg5l antibody, C88337 antibody, Paddy antibody, apg5l antibody, zgc:100934 antibody, ATATG5 antibody, AUTOPHAGY 5 antibody, MKP11.20 antibody, MKP11_20 antibody, autophagy related 5 antibody, Autophagy-related 5 antibody, ATG5 autophagy related 5 homolog (S. cerevisiae) antibody, autophagy related 5 L homeolog antibody, similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12 antibody, autophagy protein Apg5 family antibody, ATG5 antibody, Atg5 antibody, atg5 antibody, atg5.L antibody, APG5 antibody

Gene ID: 9474

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy