Immunohistochemistry (1:50-1:100) Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS, 150 mM NaCl, pH 7.4 (50 % glycerol, 0.02 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Garat, Fankell, Erickson, Reusch, Bauer, McMurtry, Klemm: "Platelet-derived growth factor BB induces nuclear export and proteasomal degradation of CREB via phosphatidylinositol 3-kinase/Akt signaling in pulmonary artery smooth muscle cells." in: Molecular and cellular biology, Vol. 26, Issue 13, pp. 4934-48, (2006) (PubMed).
Rothem, Stark, Assaraf: "Impaired CREB-1 phosphorylation in antifolate-resistant cell lines with down-regulation of the reduced folate carrier gene." in: Molecular pharmacology, Vol. 66, Issue 6, pp. 1536-43, (2004) (PubMed).
Park, Yang, Youn, Yang, Chae, Oh, Lee, Park, Choi, Kim, Walsh et al.: "Constitutively active glycogen synthase kinase-3beta gene transfer sustains apoptosis, inhibits proliferation of vascular smooth muscle cells, and reduces neointima formation after balloon injury in ..." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 23, Issue 8, pp. 1364-9, (2003) (PubMed).
Target
CREB1
(cAMP Responsive Element Binding Protein 1 (CREB1))