Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purpose
Goat polyclonal antibody raised against synthetic peptide of SLC12A6.
Sequence
SRHIDVCSKT KE
Specificity
This antibody is expected to recognize all 6 reported isofoms (NP_598408.1, NP_005126.1, NP_0010.35959.1, NP_0010.35960.1, NP_0010.35961.1, NP_0010.35962.1, ).
Cross-Reactivity
Human
Characteristics
Antibody Reactive Against Synthetic Peptide.
Immunogen
A synthetic peptide corresponding to human SLC12A6.
ELISA (1:16000) Western blot (0.1-0.3 μg/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 μg/mL) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.5 mg/mL
Buffer
In Tris saline, pH 7.3 (0.5 % BSA, 0.02 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Simard, Bergeron, Frenette-Cotton, Carpentier, Pelchat, Caron, Isenring: "Homooligomeric and heterooligomeric associations between K+-Cl- cotransporter isoforms and between K+-Cl- and Na+-K+-Cl- cotransporters." in: The Journal of biological chemistry, Vol. 282, Issue 25, pp. 18083-93, (2007) (PubMed).
Target
SLC12A6
(Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 6 (SLC12A6))