RHOA antibody (pSer188)

Catalog No. ABIN548452

Product Details anti-RHOA Antibody

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Binding Specificity: pSer188

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of RHOA.

Specificity: This peptide sequence is highly conserved in rat and mouse RhoA, but is not found in RhoB or RhoC proteins.

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S188 of human RHOA.

Application Details

Application Notes: ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (50 % glycerol, 1 mg/mL BSA, 0.05 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-RHOA antibody (ABIN548452)

Ellerbroek, Wennerberg, Burridge: "Serine phosphorylation negatively regulates RhoA in vivo." in: The Journal of biological chemistry, Vol. 278, Issue 21, pp. 19023-31, (2003) (PubMed).

Sawada, Itoh, Yamashita, Doi, Inoue, Masatsugu, Fukunaga, Sakaguchi, Sone, Yamahara, Yurugi, Nakao: "cGMP-dependent protein kinase phosphorylates and inactivates RhoA." in: Biochemical and biophysical research communications, Vol. 280, Issue 3, pp. 798-805, (2001) (PubMed).

Target Details for RHOA

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Alternative Name: RhoA (RHOA Products)

Synonyms: ARH12 antibody, ARHA antibody, RHO12 antibody, RHOH12 antibody, Arha antibody, Arha1 antibody, Arha2 antibody, arha antibody, arha2 antibody, xrhoa antibody, arh12 antibody, rho12 antibody, rhoh12 antibody, rhoa antibody, RHO1 antibody, Rhoa antibody, wu:fa66g04 antibody, wu:fb26g07 antibody, zgc:66058 antibody, zgc:85805 antibody, ARHC antibody, rhoae antibody, zgc:64015 antibody, ras homolog family member A antibody, ras homolog family member A S homeolog antibody, ras homolog family member A L homeolog antibody, ras homolog gene family, member Aa antibody, ras homolog family member Ca antibody, RHOA antibody, Rhoa antibody, rhoa.S antibody, rhoa antibody, rhoa.L antibody, rhoaa antibody, rhoca antibody

Gene ID: 387

Pathways: Microtubule Dynamics, WNT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, SARS-CoV-2 Protein Interactome