BNIP3 antibody (C-Term)

Catalog No. ABIN548542

Product Details anti-BNIP3 Antibody

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BNIP3 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit polyclonal antibody raised against synthetic peptide of BNIP3.

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Immunogen: A synthetic peptide corresponding to amino acids at C-terminus of human BNIP3.

Isotype: IgG

Application Details

Application Notes: Western Blot (1-2 μg/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 μg/mL)
Immunohistochemistry (Frozen sections) (0.5-1 μg/mL)
Immunocytochemistry (0.5-1 μg/mL)
The optimal working dilution should be determine

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from 0.9 mg NaCl, 0.2 mg Na₂HPO₄ (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.

References for anti-BNIP3 antibody (ABIN548542)

Burton, Henson, Baijal, Eisenstat, Gibson: "The pro-cell death Bcl-2 family member, BNIP3, is localized to the nucleus of human glial cells: Implications for glioblastoma multiforme tumor cell survival under hypoxia." in: International journal of cancer. Journal international du cancer, Vol. 118, Issue 7, pp. 1660-9, (2006) (PubMed).

Ray, Chen, Vande Velde, Cizeau, Park, Reed, Gietz, Greenberg: "BNIP3 heterodimerizes with Bcl-2/Bcl-X(L) and induces cell death independent of a Bcl-2 homology 3 (BH3) domain at both mitochondrial and nonmitochondrial sites." in: The Journal of biological chemistry, Vol. 275, Issue 2, pp. 1439-48, (2000) (PubMed).

Target Details for BNIP3

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Alternative Name: NIP3 (BNIP3 Products)

Synonyms: NIP3 antibody, Nip3 antibody, BCL2 interacting protein 3 antibody, BCL2/adenovirus E1B interacting protein 3 antibody, BNIP3 antibody, Bnip3 antibody

Gene ID: 664

Pathways: Autophagy, Brown Fat Cell Differentiation