PDPK1 antibody (pSer396)

Catalog No. ABIN548669

Product Details anti-PDPK1 Antibody

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Binding Specificity: pSer396

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: ELISA, Dot Blot (DB)

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of PDPK1.

Cross-Reactivity: Human

Immunogen: Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S396 of human PDPK1.

Application Details

Application Notes: ELISA (1:1000)
Dot Blot (1:500)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-PDPK1 antibody (ABIN548669)

Nilsen, Slagsvold, Skjerpen, Brech, Stenmark, Olsnes: "Peroxisomal targeting as a tool for assaying potein-protein interactions in the living cell: cytokine-independent survival kinase (CISK) binds PDK-1 in vivo in a phosphorylation-dependent manner." in: The Journal of biological chemistry, Vol. 279, Issue 6, pp. 4794-801, (2004) (PubMed).

Collins, Deak, Arthur, Armit, Alessi: "In vivo role of the PIF-binding docking site of PDK1 defined by knock-in mutation." in: The EMBO journal, Vol. 22, Issue 16, pp. 4202-11, (2003) (PubMed).

Egawa, Maegawa, Shi, Nakamura, Obata, Yoshizaki, Morino, Shimizu, Nishio, Suzuki, Kashiwagi et al.: "Membrane localization of 3-phosphoinositide-dependent protein kinase-1 stimulates activities of Akt and atypical protein kinase C but does not stimulate glucose transport and glycogen synthesis in ..." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38863-9, (2002) (PubMed).

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDPK1 (PDPK1 Products)

Synonyms: pdpk1 antibody, MGC82080 antibody, PDK1 antibody, PDPK2 antibody, PRO0461 antibody, Pdk1 antibody, zgc:153787 antibody, 3'-phosphoinositide-dependent protein kinase 1 antibody, 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 antibody, ATPDK1 antibody, AtPDK1 antibody, T32M21.110 antibody, T32M21_110 antibody, zgc:77318 antibody, 3-phosphoinositide dependent protein kinase 1 antibody, 3-phosphoinositide dependent protein kinase 1 L homeolog antibody, 3-phosphoinositide dependent protein kinase-1 antibody, 3-phosphoinositide dependent protein kinase 1a antibody, 3-phosphoinositide-dependent protein kinase 1 antibody, 3'-phosphoinositide-dependent protein kinase 1 antibody, 3-phosphoinositide dependent protein kinase 1b antibody, PDPK1 antibody, pdpk1.L antibody, pdpk1 antibody, Pdpk1 antibody, pdpk1a antibody, LOC100380750 antibody, pdk-1 antibody, PDK1 antibody, pdpk1b antibody

Gene ID: 5170

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals