Western Blot (1:1000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50 % glycerol, 10 % BSA)
Storage
-20 °C
Storage Comment
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Liu, Lewellyn, Chen, Maller: "The polo box is required for multiple functions of Plx1 in mitosis." in: The Journal of biological chemistry, Vol. 279, Issue 20, pp. 21367-73, (2004) (PubMed).
Erikson, Haystead, Qian, Maller: "A feedback loop in the polo-like kinase activation pathway." in: The Journal of biological chemistry, Vol. 279, Issue 31, pp. 32219-24, (2004) (PubMed).
van Vugt, van de Weerdt, Vader, Janssen, Calafat, Klompmaker, Wolthuis, Medema: "Polo-like kinase-1 is required for bipolar spindle formation but is dispensable for anaphase promoting complex/Cdc20 activation and initiation of cytokinesis." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36841-54, (2004) (PubMed).