GAD65 antibody (N-Term)

Catalog No. ABIN5514805

The Rabbit Polyclonal anti-GAD65 antibody is suitable to detect GAD65 in samples from Human. It has been validated for WB.

Quick Overview for GAD65 antibody (N-Term) (ABIN5514805)

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAD65 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GAD65 Antibody

Binding Specificity: N-Term

Purpose: GAD2 Antibody - N-terminal region

Sequence: FGSEDGSGDS ENPGTARAWC QVAQKFTGGI GNKLCALLYG DAEKPAESGG

Characteristics: This is a rabbit polyclonal antibody against DCE2. It was validated on Western Blot.

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human DCE2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP74935-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for GAD65

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Alternative Name: GAD2

Background:

Background Information: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Gene Name: glutamate decarboxylase 2

Alternative Symbols: GAD65

Protein Name: glutamate decarboxylase 2

Molecular Weight: 64kDa

Gene ID: 2572

NCBI Accession: NP_001127838

UniProt: Q05329