PCNA antibody (N-Term)

Catalog No. ABIN5515025

This anti-PCNA antibody is a Rabbit Polyclonal antibody detecting PCNA in WB. Suitable for Human.

Quick Overview for PCNA antibody (N-Term) (ABIN5515025)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PCNA Antibody

Binding Specificity: N-Term

Sequence: AMGVNLTSMS KILKCAGNED IITLRAEDNA DTLALVFEAP NQEKVSDYEM

Characteristics: This is a rabbit polyclonal antibody against PCNA. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human PCNA

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Alias Symbols: PCNA,

Protein Interaction Partner: GADD45A, CDKN1A, HUWE1, POLH, PCNA, MSH3, FUS, UBC, TMEM218, CHFR, DTL, POLE, POLD1, MCM7, MCM4, LIG1, MSH6, FEN1, FBXO18, ZRANB3, CHTF18, DNMT1, RAD18, SIVA1, KIAA0101, TDG, SUMO2, SUMO3, RPA1, RFC5, RFC4, RFC3, RFC2, RFC1, MDM2, TRIM25, USP18, ISG15, MA

Protein Size: 261

Gene ID: 5111

NCBI Accession: NP_872590

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy