COX1 antibody (C-Term)

Catalog No. ABIN5518658

Product Details anti-COX1 Antibody

Target: COX1 (Cytochrome C Oxidase Subunit I (COX1))

Binding Specificity: AA 501-514, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Cytochrome c oxidase subunit 1(MT-CO1) detection. Tested with WB, IHC-P, ICC in Human.

Sequence: PYHTFEEPVY MKS

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Cytochrome c oxidase subunit 1(MT-CO1) detection. Tested with WB, IHC-P, ICC in Human.
Gene Name: mitochondrially encoded cytochrome c oxidase I
Protein Name: Cytochrome c oxidase subunit 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MTCO1(501-514aa PYHTFEEPVYMKS).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for COX1

Target: COX1 (Cytochrome C Oxidase Subunit I (COX1))

Alternative Name: MT-CO1 (COX1 Products)

Synonyms: COX-1 antibody, COI antibody, MTCO1 antibody, COX1 antibody, CoxI antibody, prostaglandin-endoperoxide synthase 1 antibody, cytochrome c oxidase subunit I antibody, cytochrome c oxidase subunit 1 antibody, cytochrome c oxidase 1 antibody, PTGS1 antibody, COX1 antibody, cox1 antibody

Background: Cytochrome c oxidase subunit I(CO1 or MTCO1) is 1 of 3 mitochondrial DNA(mtDNA) encoded subunits(MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III(MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al.(2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase(COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant, cultured cells can maintain several mtDNA haplotypes at stable frequencies, the respiratory chain has little spare COX capacity, and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.

Synonyms: COI antibody|COX I antibody|COX1_HUMAN antibody|COXI antibody|Cytochrome c oxidase polypeptide I antibody|Cytochrome c oxidase subunit 1 antibody|Cytochrome C Oxidase subunit I antibody|Mitochondrially encoded cytochrome c oxidase I antibody|MT CO1 antibody|MT-CO1 antibody|MTCO 1 antibody|MTCO1 antibody

UniProt: P00395

Pathways: Proton Transport