HSD11B1 antibody (N-Term)

Catalog No. ABIN5518666

Product Details anti-HSD11B1 Antibody

Target: HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))

Binding Specificity: AA 16-33, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD11B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Corticosteroid 11-beta-dehydrogenase isozyme 1(HSD11B1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: MAYYYYSANE EFRPEMLQ

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Corticosteroid 11-beta-dehydrogenase isozyme 1(HSD11B1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: hydroxysteroid(11-beta) dehydrogenase 1
Protein Name: Corticosteroid 11-beta-dehydrogenase isozyme 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HSD11B1(16-33aa MAYYYYSANEEFRPEMLQ), different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for HSD11B1

Target: HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))

Alternative Name: HSD11B1 (HSD11B1 Products)

Synonyms: 11-DH antibody, 11-beta-HSD1 antibody, CORTRD2 antibody, HDL antibody, HSD11 antibody, HSD11B antibody, HSD11L antibody, SDR26C1 antibody, hsd11 antibody, hsd11b antibody, LRRGT00065 antibody, hydroxysteroid 11-beta dehydrogenase 1 antibody, hydroxysteroid (11-beta) dehydrogenase 1b antibody, hydroxysteroid (11-beta) dehydrogenase 1 L homeolog antibody, HSD11B1 antibody, HSD11B1b antibody, hsd11b1.L antibody, Hsd11b1 antibody

Background: 11beta-hydroxysteroid dehydrogenase type 1 is an NADPH-dependent enzyme highly expressed in key metabolic tissues including liver, adipose tissue, and the central nervous system. In these tissues, HSD11B1 reduces cortisone to the active hormone cortisol that activates glucocorticoid receptors. It is inhibited by carbenoxolone, a drug typically used in the treatment of peptic ulcers. Tannin et al.(1991) localized the HSD11B1 gene to chromosome 1. The localization was confirmed by isolating the gene from a chromosome 1-specific library using the cDNA as a probe. Schutte et al.(2000) mapped the HSD11B1 gene to 1q32-q41.

Synonyms: 11 beta HSD 1 antibody|11 beta HSD1 antibody|11 beta hydroxysteroid dehydrogenase 1 antibody|11 DH antibody|11-beta hydroxysteroid dehydrogenase, type 1 antibody|11-beta-HSD1 antibody|11-beta-hydroxysteroid dehydrogenase 1 antibody|11-DH antibody|11DH antibody|Corticosteroid 11 beta dehydrogenase isozyme 1 antibody|Corticosteroid 11-beta-dehydrogenase isozyme 1 antibody|CORTRD2 antibody|DHI1_HUMAN antibody|HDL antibody|HSD 11 antibody|HSD11 antibody|HSD11B antibody|HSD11B1 antibody|HSD11L antibody|Hydroxysteroid(11 beta) dehydrogenase 1 antibody|Hydroxysteroid(11 beta) dehydrogenase antibody|MGC13539 antibody|SDR26C1 antibody|short chain dehydrogenase/reductase family 26C, member 1 antibody

UniProt: P28845

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Carbohydrate Metabolic Process