Cytokeratin 18 antibody (AA 204-430)

Catalog No. ABIN5518769

Product Details anti-Cytokeratin 18 Antibody

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Binding Specificity: AA 204-430

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Keratin, type I cytoskeletal 18(KRT18) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Keratin, type I cytoskeletal 18(KRT18) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: keratin 18
Protein Name: Keratin, type I cytoskeletal 18

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Cytokeratin 18 recombinant protein (Position: E204-H430). Human Cytokeratin 18 shares 87.7% and 85.9% amino acid (aa) sequence identity with mouse and rat Cytokeratin 18, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Cytokeratin 18 antibody (ABIN5518769)

Li, Li, Sun, Wu, Wang: "Comparison of the effect of recombinant bovine wild and mutant lipopolysaccharide-binding protein in lipopolysaccharide-challenged bovine mammary epithelial cells." in: Cell stress & chaperones, Vol. 21, Issue 3, pp. 439-52, (2017) (PubMed).

Zhang, Liu, Liu, Zhou, Song, Cao, An: "miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression." in: PLoS ONE, Vol. 12, Issue 7, pp. e0179783, (2017) (PubMed).

Wu, Guo, Hui, Ye, Xiang, Wan, Zou, Ma, Sun, Yang, Guo: "Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage." in: Journal of veterinary science, Vol. 15, Issue 4, pp. 475-83, (2015) (PubMed).

Xu, Jin, Min, Li, Deng, Wu, Lin, Chen, Zhang, Li, Wang, Zhu, Wang, Chu, Shen, Li, Mao: "Chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival." in: Oncotarget, Vol. 6, Issue 4, pp. 2434-50, (2015) (PubMed).

Yao, Guo, Wang, Xu, Zhang, Li, Lai: "The Paracrine Effect of Transplanted Human Amniotic Epithelial Cells on Ovarian Function Improvement in a Mouse Model of Chemotherapy-Induced Primary Ovarian Insufficiency." in: Stem cells international, Vol. 2016, pp. 4148923, (2015) (PubMed).

Hu, Zhou, Shi, Zhu, Wang, Zhang, Hu, Qian, Xu: "Mesenchymal stem-like cells isolated from human esophageal carcinoma and adjacent non-cancerous tissues." in: Oncology letters, Vol. 5, Issue 1, pp. 179-184, (2012) (PubMed).

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: KRT18 (KRT18 Products)

Synonyms: CK18 antibody, K18 antibody, Krt1-18 antibody, CYK18 antibody, DreK18 antibody, cb83 antibody, dapk1 antibody, sb:cb83 antibody, wu:fa13f02 antibody, wu:fb36b04 antibody, krt18 antibody, MGC64569 antibody, MGC75922 antibody, KRT18 antibody, CK-18-A antibody, K18-A antibody, NC-11 antibody, cyk18 antibody, k18 antibody, krt18-a antibody, krt18-b antibody, krt18a antibody, xkendob antibody, keratin 18 antibody, keratin 18, type I L homeolog antibody, keratin 18, type I antibody, keratin, type I cytoskeletal 18 antibody, K18, simple type I keratin antibody, Keratin, type I cytoskeletal 18 antibody, keratin 18, type I S homeolog antibody, Krt18 antibody, KRT18 antibody, krt18 antibody, krt18.L antibody, LOC700569 antibody, LOC100136778 antibody, k1c18 antibody, LOC100008885 antibody, krt18.S antibody

Background: Keratin 18, mapped to 12q13.13, is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Synonyms: CK18 | CK-18 | CK 18 | CYK 18 | CYK18 | Cytokeratin 18 | Cytokeratin endo B | Cytokeratin-18 | K 18 | K18 | KA18 | Keratin 18 | Keratin D | Keratin-18 | Krt18 | P05783

Gene ID: 3875

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis