Nephrin antibody (AA 1084-1241)

Catalog No. ABIN5518783

Product Details anti-Nephrin Antibody

Target: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Binding Specificity: AA 1084-1241

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nephrin antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Nephrin(NPHS1) detection. Tested with WB in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Nephrin(NPHS1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: nephrosis 1, congenital, Finnish type (nephrin)
Protein Name: Nephrin

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Nephrin recombinant protein (Position: V1084-V1241). Human Nephrin shares 79.9% and 75.3% amino acid (aa) sequence identity with mouse and rat Nephrin, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Nephrin antibody (ABIN5518783)

Chen, Chen, Liu, Ma: "Effect of simvastatin on the expression of nephrin, podocin, and vascular endothelial growth factor (VEGF) in podocytes of diabetic rat." in: International journal of clinical and experimental medicine, Vol. 8, Issue 10, pp. 18225-34, (2016) (PubMed).

Hao, Pan, Zheng, Wang: "Effect of Cordyceps sinensis and Tripterygium wilfordii polyglycosidium on podocytes in rats with diabetic nephropathy." in: Experimental and therapeutic medicine, Vol. 7, Issue 6, pp. 1465-1470, (2014) (PubMed).

Target Details for Nephrin

Target: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Alternative Name: NPHS1 (NPHS1 Products)

Synonyms: NPHS1 antibody, nphs1l antibody, nphs1 antibody, CNF antibody, NPHN antibody, nephrin antibody, NephrinB antibody, NPHS1, nephrin antibody, nephrosis 1, congenital, Finnish type (nephrin) antibody, NPHS1 nephrin S homeolog antibody, nephrin antibody, NPHS1 nephrin antibody, nephrosis 1, nephrin antibody, NPHS1 antibody, nphs1 antibody, nphs1.S antibody, LOC5579127 antibody, Nphs1 antibody

Background: NPHS1, also called Nephrin, is mapped to 19q13.12. This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. It gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. A defect in the gene for nephrin, NPHS1, is associated with congenital nephrotic syndrome of the Finnish type and causes massive amounts of protein to be leaked into the urine, orproteinuria. NPHS1 is also required for cardiovascular development.

Synonyms: CNF | Nephrin | NPHN | NPHS 1 | NPHS1 | O60500

Gene ID: 4868

UniProt: O60500

Pathways: Regulation of Actin Filament Polymerization, Skeletal Muscle Fiber Development