CHEK1 antibody (AA 1-210)

Catalog No. ABIN5518819

The Rabbit Polyclonal anti-CHEK1 antibody is suitable to detect CHEK1 in samples from Human. It has been validated for WB and FACS.

Quick Overview for CHEK1 antibody (AA 1-210) (ABIN5518819)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-CHEK1 Antibody

Binding Specificity: AA 1-210

Purpose: Anti-Chk1/CHEK1 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human Chk1 shares 96.7%,97.6% amino acid (aa) sequence identity with mouse,rat Chk1,respectively.

Characteristics: Anti-Chk1/CHEK1 Antibody. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Chk1 recombinant protein (Position: M1-Q210). Human Chk1 shares 96.7% and 97.6% amino acid (aa) sequence identity with mouse and rat Chk1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHEK1

Background:

Background: CHEK1, Cell cycle checkpoint kinase, is an enzyme that in humans is encoded by the CHEK1 gene. By fluorescence in situ hybridization, the human CHEK1 gene is mapped to 11q24, near the ATM gene at 11q23. CHEK1 is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Furthermore, CHEK1 acts to integrate signals from ATM and ATR, and is involved in monitoring meiotic recombination, a process that involves programmed DNA breaks.

Gene Full Name: checkpoint kinase 1

Molecular Weight: 54 kDa

Gene ID: 1111

UniProt: O14757

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair