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RAF1 antibody (AA 364-451)

RAF1 Reactivity: Human, Mouse, Rat WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5518949
  • Target See all RAF1 Antibodies
    RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
    Binding Specificity
    • 48
    • 43
    • 35
    • 34
    • 31
    • 27
    • 24
    • 23
    • 23
    • 21
    • 19
    • 17
    • 15
    • 13
    • 13
    • 8
    • 8
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 364-451
    Reactivity
    • 439
    • 227
    • 155
    • 14
    • 12
    • 12
    • 12
    • 9
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 471
    • 14
    • 2
    • 2
    Rabbit
    Clonality
    • 449
    • 40
    Polyclonal
    Conjugate
    • 194
    • 37
    • 28
    • 27
    • 17
    • 16
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    This RAF1 antibody is un-conjugated
    Application
    • 291
    • 212
    • 105
    • 104
    • 89
    • 73
    • 47
    • 31
    • 28
    • 24
    • 16
    • 11
    • 8
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB)
    Purpose
    Rabbit IgG polyclonal antibody for RAF proto-oncogene serine/threonine-protein kinase(RAF1) detection. Tested with WB in Human,Mouse,Rat.
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for RAF proto-oncogene serine/threonine-protein kinase(RAF1) detection. Tested with WB in Human,Mouse,Rat.
    Gene Name: Raf-1 proto-oncogene, serine/threonine kinase
    Protein Name: RAF proto-oncogene serine/threonine-protein kinase
    Purification
    Immunogen affinity purified.
    Immunogen
    E. coli-derived human Raf1 recombinant protein (Position: Y364-Q451). Human Raf1 shares 98.9% amino acid (aa) sequence identity with both mouse and rat Raf1.
    Isotype
    IgG
    Top Product
    Discover our top product RAF1 Primary Antibody
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
    Notes: Tested Species: Species with positive results.
    Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Zhao, Lin, Hu, Yin, Wang, Xu, Li: "The role of Dectin-1/Raf-1 signal cascade in innate immune of human corneal epithelial cells against Aspergillus fumigatus infection." in: International journal of ophthalmology, Vol. 9, Issue 10, pp. 1371-1375, (2016) (PubMed).

  • Target
    RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
    Alternative Name
    RAF1 (RAF1 Products)
    Synonyms
    CRAF antibody, NS5 antibody, Raf-1 antibody, c-Raf antibody, c-raf antibody, craf antibody, raf1 antibody, RAF1 antibody, 6430402F14Rik antibody, AA990557 antibody, BB129353 antibody, Craf1 antibody, D830050J10Rik antibody, v-Raf antibody, raf antibody, cRaf antibody, Raf-1 proto-oncogene, serine/threonine kinase antibody, Raf-1 proto-oncogene, serine/threonine kinase a antibody, v-raf-leukemia viral oncogene 1 antibody, Raf-1 proto-oncogene, serine/threonine kinase S homeolog antibody, RAF1 antibody, Raf1 antibody, raf1a antibody, raf1 antibody, raf1.S antibody
    Background
    RAF proto-oncogene serine/threonine-protein kinase, also known as proto-oncogene c-RAF or simply c-Raf or even Raf-1, is an enzyme that in humans is encoded by the RAF1 gene. This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinasekinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

    Synonyms: RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, RAF1, RAF
    Gene ID
    5894
    UniProt
    P04049
    Pathways
    MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
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