TRAF5 antibody (AA 277-557)

Catalog No. ABIN5518962

Product Details anti-TRAF5 Antibody

Target: TRAF5 (TNF Receptor-Associated Factor 5 (TRAF5))

Binding Specificity: AA 277-557

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAF5 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for TNF receptor-associated factor 5(TRAF5) detection. Tested with WB in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for TNF receptor-associated factor 5(TRAF5) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: TNF receptor associated factor 5
Protein Name: TNF receptor-associated factor 5

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human TRAF5 recombinant protein (Position: K277-L557). Human TRAF5 shares 82.3% amino acid (aa) sequence identity with mouse TRAF5.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for TRAF5

Target: TRAF5 (TNF Receptor-Associated Factor 5 (TRAF5))

Alternative Name: TRAF5 (TRAF5 Products)

Synonyms: MGC:39780 antibody, RNF84 antibody, TNF receptor associated factor 5 antibody, TNF receptor-associated factor 5 antibody, TRAF5 antibody, Traf5 antibody

Background: TNF receptor-associated factor 5, also known as RNF84, is a protein that in humans is encoded by the TRAF5 gene. The scaffold protein encoded by this gene is a member of the tumor necrosis factor receptor-associated factor (TRAF) protein family. Fluorescence in situ hybridization confirmed the regional localization of human TRAF5 to chromosome 1q32.2-q32.3. This protein is one of the components of a multiple protein complex which binds to tumor necrosis factor (TNF) receptor cytoplasmic domains and mediates TNF-induced activation. It is an adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. It seems to be involved in apoptosis.

Synonyms: TNF receptor-associated factor 5, RING finger protein 84, TRAF5, RNF84

Gene ID: 7188

UniProt: O00463

Pathways: NF-kappaB Signaling, Apoptosis