Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Immunogen
This MAGEA10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 324-353 amino acids from the C-terminal region of human MAGEA10.
MAGEA10
Reactivity: Human
IF (cc), IF (p)
Host: Rabbit
Polyclonal
Alexa Fluor 750
Application Notes
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Restrictions
For Research Use only
Format
Liquid
Concentration
2 mg/mL
Buffer
Supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
MAGEA10 antibody, CT1.10 antibody, MAGE10 antibody, RGD1563693 antibody, MAGEA11 antibody, MAGE family member A10 antibody, melanoma-associated antigen 10 antibody, melanoma antigen family A, 10 antibody, putative MAGE domain-containing protein MAGEA13P antibody, MAGEA10 antibody, LOC509805 antibody, Magea10 antibody, LOC492173 antibody
Background
MAGEA10 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80 % sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita.