This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This VPS25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-144 amino acids from the C-terminal region of human VPS25.
VPS25
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
Biotin
Application Notes
For WB starting dilution is: 1:1000
Restrictions
For Research Use only
Format
Liquid
Concentration
0.35 mg/mL
Buffer
Supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
VPS25
(Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))
VPS25 antibody, CG14750 antibody, Dmel\\CG14750 antibody, ESCRT-I antibody, ESCRT-II antibody, dVps25 antibody, l(2)44Db antibody, l(2)k08904 antibody, vps25 antibody, GB16268 antibody, derp9 antibody, eap20 antibody, fap20 antibody, DDBDRAFT_0189483 antibody, DDBDRAFT_0234026 antibody, DDB_0189483 antibody, DDB_0234026 antibody, 1110020N13Rik antibody, AW107467 antibody, D11Wsu68e antibody, im:6898134 antibody, zgc:162560 antibody, DERP9 antibody, EAP20 antibody, FAP20 antibody, vacuolar protein sorting 25 homolog antibody, vacuolar protein sorting 25 homolog (S. cerevisiae) pseudogene antibody, vacuolar protein sorting-associated protein 25 antibody, WNK lysine deficient protein kinase 4 antibody, Vacuolar protein sorting 25 antibody, vacuolar protein-sorting-associated protein 25 antibody, vacuolar protein sorting 25 homolog L homeolog antibody, vacuolar protein sorting 25 antibody, vacuolar protein sorting 25 homolog (S. cerevisiae) antibody, E2F/DP family winged-helix DNA-binding domain-containing protein antibody, VPS25 antibody, LOC459431 antibody, CpipJ_CPIJ010292 antibody, WNK4 antibody, Vps25 antibody, LOC412381 antibody, vps25.L antibody, vps25 antibody
Background
VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL, MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741], Kamura et al., 2001 [PubMed 11278625]).