SIGLEC8 antibody (N-Term)

Catalog No. ABIN5534687

This Rabbit Polyclonal antibody specifically detects SIGLEC8 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for SIGLEC8 antibody (N-Term) (ABIN5534687)

Target: SIGLEC8 (Sialic Acid Binding Ig-Like Lectin 8 (SIGLEC8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIGLEC8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SIGLEC8 Antibody

Binding Specificity: AA 94-122, N-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SIGLEC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-122 amino acids from the N-terminal region of human SIGLEC8.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for SIGLEC8

Target: SIGLEC8 (Sialic Acid Binding Ig-Like Lectin 8 (SIGLEC8))

Alternative Name: SIGLEC8

Background: Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM, MIM 603492) that mediates an association with SLAM-associated protein (SAP, MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).

Molecular Weight: 54 kDa

Gene ID: 27181

UniProt: Q9NYZ4