SIGLEC8 antibody (C-Term)

Catalog No. ABIN5534688

The Rabbit Polyclonal anti-SIGLEC8 antibody is suitable to detect SIGLEC8 in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for SIGLEC8 antibody (C-Term) (ABIN5534688)

Target: SIGLEC8 (Sialic Acid Binding Ig-Like Lectin 8 (SIGLEC8))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIGLEC8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SIGLEC8 Antibody

Binding Specificity: AA 373-406, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Immunogen: This SIGLEC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-406 amino acids from the C-terminal region of human SIGLEC8.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.67 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for SIGLEC8

Target: SIGLEC8 (Sialic Acid Binding Ig-Like Lectin 8 (SIGLEC8))

Alternative Name: SIGLEC8

Background: SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3-linked sialic acid. and also binds to alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC8 is expressed specifically on eosinophils. The protein contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The SIGLEC8 gene belongs to the immunoglobulin superfamily.

Molecular Weight: 54 kDa

Gene ID: 27181

UniProt: Q9NYZ4