POLR3G antibody (C-Term)

Catalog No. ABIN5539104

Product Details anti-POLR3G Antibody

Target: POLR3G (Polymerase (RNA) III (DNA Directed) Polypeptide G (32kD) (POLR3G))

Binding Specificity: AA 195-223, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3G antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This POLR3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-223 amino acids from the C-terminal region of human POLR3G.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for POLR3G

Target: POLR3G (Polymerase (RNA) III (DNA Directed) Polypeptide G (32kD) (POLR3G))

Alternative Name: POLR3G (POLR3G Products)

Synonyms: 2310047G20Rik antibody, AV275904 antibody, RPC32 antibody, RPC7 antibody, polymerase (RNA) III (DNA directed) polypeptide G antibody, RNA polymerase III subunit G antibody, Polr3g antibody, POLR3G antibody

Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the RPC3/POLR3C-RPC6/POLR3F-RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.

Molecular Weight: 26 kDa

Gene ID: 10622

UniProt: O15318