Rabbit anti-Sheep IgM (Heavy & Light Chain) Antibody

Catalog No. ABIN5541681

Product Details

Target: IgM

Binding Specificity: Heavy & Light Chain

Reactivity: Sheep

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Dot Blot (DB), Enzyme Immunoassay (EIA)

Purification: Hyperimmune antisera with strong activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Purified normal IgM isolated from pooled sheep serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Precipitin titre : 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2 Preservatives: None, as it may interfere with the antibody activity. Stabilizers: None

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody