FKBP4 antibody

Catalog No. ABIN452368

Product Details anti-FKBP4 Antibody

Target: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Reactivity: Human, Mouse, Rat, Hamster, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This FKBP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects an ~52 kDa protein representing FKBP52 in HeLa cell lysate. Also detects FKBP52 in whole tissue extracts from Rat kidney and Rat and Mouse testes. Heavy chain migrates close to FKBP52 on SDS-PAGE.

Cross-Reactivity (Details): Species reactivity (tested):Canine, Hamster, Human, Mouse, Rat

Characteristics: Synonyms: FK506-binding protein 4, FKBP52 protein, FKBP59, p59 protein, Peptidyl-prolyl cis-transisomerase, PPIase, HSP-binding immunophilin, HBI, 52 kDa FK506-binding protein,Rotamase

Purification: Protein G Chromatography

Immunogen: Synthetic peptide corresponding to the residues of human FKBP52

Clone: Hi52C

Isotype: IgG1

Application Details

Application Notes: Western blot (1): 1: 2000. Immunoprecipitation (1): 5 μg with 10-20 μL Protein A beads. Imunohistochemistry on paraffin embedded sections (1): 1: 250.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS in 50 % Glycerol, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for FKBP4

Target: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Alternative Name: FKBP4 (FKBP4 Products)

Synonyms: HBI antibody, p52 antibody, hsp56 antibody, PPIase antibody, fkbp52 antibody, fkbp59 antibody, MGC81078 antibody, wu:fb34f09 antibody, 59kDa antibody, AL022792 antibody, AW208983 antibody, FKBP-4 antibody, FKBP-52 antibody, FKBP52 antibody, FKPB52 antibody, p59 antibody, FKBP51 antibody, FKBP59 antibody, Hsp56 antibody, P59 antibody, FK506 binding protein 4 antibody, FK506 binding protein 4 S homeolog antibody, FKBP4 antibody, fkbp4.S antibody, fkbp4 antibody, Fkbp4 antibody

Background: Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin Abinding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90 (4). Although FKBP52 and FKBP51 share ~75 % sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90-binding characteristics (3, 5). Also, whereas FKBP51 typically has a role with the progesterone receptor, FKBP52 has been found to be linked to the progesterone, androgen and glucocorticoid receptors (5).Synonyms: 52 kDa FK506-binding protein, FK506-binding protein 4, FKBP52 protein, FKBP59, HBI, HSP-binding immunophilin, PPIase, Peptidyl-prolyl cis-trans isomerase, Rotamase, p59 protein

Gene ID: 2288

UniProt: Q02790

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway