This Tryptophan Hydroxylase 1 antibody is un-conjugated
Application
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
Specificity
Specific for the ~55k Tryptophan Hydroxylase protein. The antibody has been directly tested for reactivity in Western blots with Human and Rat tissue. Based upon the relatively high degree of homology of Tryptophan Hydroxylase, the antibodies should cross-react with other mammalian species. Does not recognize TPH in Rabbit tissues.
Cross-Reactivity (Details)
Species reactivity (tested):Human and Rat.
Purification
Affinity Chromatography.
Immunogen
Recombinant rabbit Tryptophan Hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
TPH1
Reactivity: Human, Mouse, Rat
WB, ELISA, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western blotting: 1/1,000. Immunohistochemistry on Frozen Sections: 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Buffer
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store the antibody undiluted (in aliquots) at-20 °C.
Tryptophan Hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001).Synonyms: TPRH, TRPH, Tryptophan 5-monooxygenase 1