MYL6 antibody (AA 12-27)
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- Target See all MYL6 Antibodies
- MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))
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Binding Specificity
- AA 12-27
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MYL6 antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificity
- This antibody is directed against the regulatory light chain of smooth and nonmuscle Myosin. The antibody detects both unphosphorylated and monophosphorylated forms of the protein. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
- Purification
- Immunoaffinity Chromatography.
- Immunogen
- Synthetic peptide corresponding to amino acids 12-27 of Human Myosin light chain protein.
- Isotype
- IgG
- Top Product
- Discover our top product MYL6 Primary Antibody
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- Application Notes
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Tested in ELISA (1/5000-1/20000) and Western Blot (1/500-1/2000). Although not tested, this antibody is likely functional in Immunohistochemistry andImmunoprecipitation (1/100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.71 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Target
- MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))
- Alternative Name
- MYL6 (MYL6 Products)
- Synonyms
- ESMLC antibody, LC17 antibody, LC17-GI antibody, LC17-NM antibody, LC17A antibody, LC17B antibody, MLC-3 antibody, MLC1SM antibody, MLC3NM antibody, MLC3SM antibody, zgc:103624 antibody, MLC3nm antibody, Myln antibody, myosin light chain 6 antibody, myosin, light chain 6, alkali, smooth muscle and non-muscle antibody, myosin light chain 6 L homeolog antibody, myosin, light polypeptide 6, alkali, smooth muscle and non-muscle antibody, MYL6 antibody, Myl6 antibody, myl6 antibody, myl6.L antibody
- Background
- Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human, mouse and rat have almost identical sequences.Synonyms: 17 kDa myosin light chain, LC17, MLC-3, Myosin light chain 3, Myosin light chain alkali 3, Myosin light polypeptide 6, Smooth muscle and nonmuscle myosin light chain alkali 6
- Gene ID
- 4637
- NCBI Accession
- NP_066299
- UniProt
- P60660
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