Peptide ELISA: Antibody Detection Limit Dilution: 1/16000. Western Blot: 0.1-0.3 μg/mL. Approx 70 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of42.0 kDa according to NP_660339.1). The observed molecular weight corresponds tofindings with anti-CADM4 antibody (C-term) ABIN374902, a product of different designreacting to a different part of the same protein. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Heffernan, Jain, Liu, Kim, Barretto, Li, Maurel: "Nectin-like 4 Complexes with Choline Transporter-like Protein-1 and Regulates Schwann Cell Choline Homeostasis and Lipid Biogenesis in Vitro." in: The Journal of biological chemistry, Vol. 292, Issue 11, pp. 4484-4498, (2017) (PubMed).
Cell Adhesion Molecule 4 (CADM4) is involved in the cell-cell adhesion. It has calcium and magnesium independent cell-cell adhesion activity and may have tumor suppressor activity.Synonyms: Cell adhesion molecule 4, IGSF4C, Immunoglobulin superfamily member 4C, NECL4, Nectin-like protein 4, TSLC1-like protein 2, TSLL2