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VPS35 antibody (C-Term)
VPS35
Reactivity: Human, Mouse, Rat, Cow
WB, IF, IHC (p), EIA
Host: Goat
Polyclonal
unconjugated
Product Details anti-VPS35 Antibody
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Target
See all VPS35 Antibodies
VPS35
(Vacuolar Protein Sorting 35 (VPS35))
Binding Specificity
All epitopes for VPS35 antibodies
C-Term
Reactivity
All reactivities for VPS35 antibodies
Human, Mouse, Rat, Cow
Host
All hosts for VPS35 antibodies
Goat
Clonality
All clonalities for VPS35 antibodies
Polyclonal
Conjugate
All conjugates for VPS35 antibodies
This VPS35 antibody is un-conjugated
Application
All applications for VPS35 antibodies
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
Sequence
C-SPESEGPIYE GLIL
Specificity
This antibody detects VPS35 / MEM3 at C-term.
Cross-Reactivity (Details)
Species reactivity (expected):Mouse, Rat, Cow. Species reactivity (tested):Human.
Purification
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Immunogen
Syntehtic peptide from the C-Terminus of Human VPS35 (NP_060676.2). Genename: VPS35
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Discover our top product VPS35 Primary Antibody
Alternatives
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Application Details
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Application Notes
Peptide ELISA: 1/16000. Western blot: Approx 90 kDa band observed in Human Brain and Testis lysates (predictedMW of 90 kDa according to NP_060676.2). Recommended concentration: 1 - 3 μg/mL. Immunohistochemistry on paraffin sections: Human Testis, shows positive staining inLeydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymisand of the rete testis. Immunoflurescence: Staining of VPS35 (red) in human melanoma cells 1205LU: Please seeScott KL et al. , Nature.2009 Jun 25,459(7250): 1085-90. , PMID: 19553991. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
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Concentration
0.5 mg/mL
Buffer
Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target Details for VPS35
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Target
VPS35
(Vacuolar Protein Sorting 35 (VPS35))
Alternative Name
VPS35 / MEM3 (VPS35 Products )
Synonyms
MEM3 antibody, PARK17 antibody, vps35 antibody, MGC81959 antibody, bZ34G2.3 antibody, si:rp71-34g2.3 antibody, wu:fa66e03 antibody, wu:fb95c08 antibody, zgc:136268 antibody, 23.m05999 antibody, Mem3 antibody, VPS35 antibody, DKFZp459E1510 antibody, CG5625 antibody, DVps35 antibody, Dmel\\CG5625 antibody, Dvps35 antibody, dvps35 antibody, AI647796 antibody, mem3 antibody, VPS35, retromer complex component antibody, VPS35 retromer complex component L homeolog antibody, VPS35 retromer complex component antibody, vacuolar protein sorting 35 homolog (S. cerevisiae) antibody, vacuolar protein sorting-associated protein 35 antibody, vacuolar protein sorting 35 antibody, Vacuolar protein sorting 35 antibody, hypothetical protein antibody, VPS35 retromer complex component S homeolog antibody, retromer complex subunit Vps35 antibody, VPS35 antibody, vps35.L antibody, vps35 antibody, Tc00.1047053511367.170 antibody, Tb10.70.5460 antibody, BBOV_IV009740 antibody, CC1G_12185 antibody, PTRG_08682 antibody, CMU_017400 antibody, PHATR_43830 antibody, MCYG_00751 antibody, MGYG_01212 antibody, EHI_086580 antibody, EHI_002990 antibody, EHI_041950 antibody, EHI_096520 antibody, Bm1_44045 antibody, LOC100285267 antibody, Vps35 antibody, PGTG_10018 antibody, Tsp_09825 antibody, vps35.S antibody
Background
Synonyms: Maternal-embryonic 3, TCCCTA00141, Vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35
Gene ID
55737
NCBI Accession
NP_060676
UniProt
Q96QK1
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