EIF3E antibody (C-Term)

Catalog No. ABIN569645

Product Details anti-EIF3E Antibody

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Binding Specificity: C-Term

Reactivity: Human, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This EIF3E antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-KLNQNSRSEA PN

Specificity: Recognizes EIF3E (C-term).

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Cow, Pig.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_001559.1. Genename: EIF3E

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EIF3E

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Alternative Name: EIF3E / EIF3S6 (EIF3E Products)

Synonyms: EIF3-P48 antibody, EIF3S6 antibody, INT6 antibody, eIF3-p46 antibody, 48kDa antibody, Eif3s6 antibody, Int6 antibody, eIF3-p48 antibody, eif3-p48 antibody, eif3s6 antibody, eif3s6-A antibody, eIF3e antibody, eIF3-S6 antibody, eif3s6-B antibody, EIF3SE antibody, eif3s6b antibody, im:7147439 antibody, QtsA-18056 antibody, eukaryotic translation initiation factor 3 subunit E antibody, eukaryotic translation initiation factor 3, subunit E antibody, eukaryotic translation initiation factor 3 subunit E L homeolog antibody, eukaryotic translation initiation factor 3 subunit 6 antibody, eukaryotic translation initiation factor 3 subunit E S homeolog antibody, eukaryotic translation initiation factor 3, subunit E, a antibody, Eukaryotic translation initiation factor 3 subunit E antibody, eukaryotic translation initiation factor 3, subunit E, b antibody, EIF3E antibody, Eif3e antibody, eif3e antibody, eif3e.L antibody, LOC692936 antibody, eif3e.S antibody, eif3ea antibody, eif-3.E antibody, eif3eb antibody, LOC108348260 antibody, int6 antibody

Background: EIF3E is part of the eIF3 complex, which is composed of at least 12 subunits. It binds the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. It can bind the COP9 signalosome and the 26S proteasome, possibly having regulatory functions in both protein translation and degradation. Reducing its expression by RNA interference in HeLa cells markedly alters mitosis progression and defects in spindle formation, chromosome segregation and cytokinesis are observed.Synonyms: Eukaryotic translation initiation factor 3 subunit 6, Eukaryotic translation initiation factor 3 subunit E, INT6, Viral integration site protein INT-6 homolog, eIF-3 p48

Molecular Weight: 52.2kDa (NP_001559.1)

Gene ID: 3646

NCBI Accession: NP_001559

UniProt: P60228

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C