CDK4 antibody (C-Term)

Catalog No. ABIN5557290

This Mouse Monoclonal antibody specifically detects CDK4 in WB, IF, ICC, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for CDK4 antibody (C-Term) (ABIN5557290)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2F6

Product Details anti-CDK4 Antibody

Binding Specificity: C-Term

Purpose: Cdk4 (2F6) Monoclonal Antibody

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: Recombinant human Cdk4 protein, around C-terminal 101aa.

Isotype: IgG2c

Application Details

Application Notes: WB(WB=1:500-2000), IHC-P(IHC-P=1:50-200), IHC-F(IHC-F=1:50-200), IF(IF=1:50-200), ICC/IF(ICC/IF=1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: Cdk4

Background:

Synonyms: Cyclin-dependent kinase 4, CDK4, Cell division protein kinase 4, PSK-J3

Background: Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Gene ID: 1019

UniProt: P11802

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size