AKT1/3 (pTyr437), (Tyr434) antibody

Catalog No. ABIN5572321

Product Details

Target: AKT1/3

Binding Specificity: pTyr437, Tyr434

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of human AKT1/AKT3.

Specificity: AKT1/AKT3 (phospho Y437/Y434) polyclonal antibody detects endogenous levels of human AKT1/AKT3 only when phosphorylated at tyrosine 437 and tyrosine 434.

Cross-Reactivity: Human, Mouse, Rat

Immunogen: Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y437/Y434 of human AKT1/AKT3.

Application Details

Application Notes: Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (without Mg²⁺ and Ca²⁺), 150 mM NaCl, pH 7.4 (50 % glycerol, 0.02 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Target Details

Target: AKT1/3

Alternative Name: AKT1/AKT3

Background: Full Gene Name: v-akt murine thymoma viral oncogene homolog 1
Synonyms: AKT,MGC99656,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA

Gene ID: 207, 10000

UniProt: P31749, Q9Y243