Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Dot Blot (DB)
Specificity
PAB28943 recognizes all types of amyloid oligomers, likely with a peptide backbone epitope that is common in amyloid oligomers but not in native proteins, amyloidogenic monomer or mature amyloid fibrils. A 1:1000 dilution of PAB28943 was sufficient for detection of amyloid oligomers in 10 μg of mouse brain lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cross-Reactivity
Human, Mouse, Rat
Immunogen
Synthetic molecular mimic of soluble APP oligomers.
Dot blot ELISA Immunofluorescence Immunohistochemistry (1:1000-10000) Immunoprecipitation (1:1000) Western Blot The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS pH 7.4, 50 % glycerol and 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store at 4 °C. Aliquot to avoid repeated freezing and thawing.