HLA-DRB1 antibody

Catalog No. ABIN5580005

Product Details anti-HLA-DRB1 Antibody

Target: HLA-DRB1 (Major Histocompatibility Complex, Class II, DR beta 1 (HLA-DRB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DRB1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Mouse monoclonal antibody raised against native human HLA-DRB1.

Specificity: This monoclonal antibody reacts with the beta chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4⁺ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B cell lymphoma.

Cross-Reactivity: Human

Immunogen: Activated human peripheral blood mononuclear cells.

Clone: HLA-DRB-1067

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry (0.5-1 μg/10⁶ cells in 0.1 mL)
Immunofluorescence (0.5-1 μg/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 μg/mL)
Western Blot (0.5-1 μg/mL)
The optimal working dilution should be d

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 10 mM PBS.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing.

Target Details for HLA-DRB1

Target: HLA-DRB1 (Major Histocompatibility Complex, Class II, DR beta 1 (HLA-DRB1))

Alternative Name: HLA-DRB1 (HLA-DRB1 Products)

Synonyms: MGC108235 antibody, DRB1 antibody, DRw10 antibody, HLA-DR1B antibody, HLA-DRB antibody, SS1 antibody, major histocompatibility complex, class II, DR beta 1 antibody, hla-drb1 antibody, HLA-DRB1 antibody

Background: Full Gene Name: major histocompatibility complex, class II, DR beta 1
Synonyms: DRB1,FLJ76359,HLA-DR1B,HLA-DRB,HLA-DRB1*,SS1

Gene ID: 3123

Pathways: TCR Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Cancer Immune Checkpoints