Keratin 77 antibody

Catalog No. ABIN5585209

Product Details anti-Keratin 77 Antibody

Target: Keratin 77 (KRT77)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 77 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Mouse monoclonal antibody raised against Pan Cytokeratin.

Specificity: This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19). AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.

Cross-Reactivity: Human

Immunogen: Human epidermal keratin.

Clone: AE1-AE3

Isotype: IgG1

Application Details

Application Notes: Western Blot (0.1 - 1.0 μg/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200 - 1:400)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (0.05 % BSA, 0.05 % sodium azide).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details for Keratin 77

Target: Keratin 77 (KRT77)

Alternative Name: KRT77 (KRT77 Products)

Synonyms: K1B antibody, KRT1B antibody, 4732484G22Rik antibody, K1emb antibody, Krt239 antibody, mK1b antibody, Kb39 antibody, keratin 77 antibody, KRT77 antibody, Krt77 antibody

Background: Full Gene Name: keratin 1
Synonyms: CK1,EHK1,K1,KRT1A

Gene ID: 374454, 51350