Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Fructosyl Amino Acid Oxidase antibody (HRP)

socD Reactivity: Corynebacterium WB, ELISA, IP Host: Sheep Polyclonal HRP
Catalog No. ABIN5596765
  • Target See all Fructosyl Amino Acid Oxidase (socD) products
    Fructosyl Amino Acid Oxidase (socD)
    Reactivity
    • 3
    • 2
    • 1
    Corynebacterium
    Host
    • 5
    • 1
    Sheep
    Clonality
    • 6
    Polyclonal
    Conjugate
    • 4
    • 2
    This Fructosyl Amino Acid Oxidase antibody is conjugated to HRP
    Application
    • 6
    • 5
    • 3
    • 2
    • 2
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Cross-Reactivity (Details)
    Cross reactivity against Fructosyl-Amino Acid Oxidase from other sources is unknown.
    Purification
    Fructosyl-amino acid oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Fructosyl-Amino Acid Oxidase [E.coli].
    Immunogen

    Immunogen: Fructosyl-Amino Acid Oxidase [from Corynebacterium sp. Expressed in E.coli]

    Immunogen Type: Recombinant Protein

    Isotype
    IgG
  • Application Notes

    Application Note: Anti-Fructosyl-amino acid oxidase has been assayed against 1.0 μg of Fructosyl-Amino Acid Oxidase [E.coli] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    Fructosyl Amino Acid Oxidase (socD)
    Alternative Name
    Fructosyl-Amino Acid Oxidase (socD Products)
    Synonyms
    fructosyl amino acid oxidase antibody, hypothetical protein antibody, socD antibody, CNB00220 antibody, AFUA_8G06440 antibody, AFUA_6G03440 antibody, PTRG_01708 antibody, ANI_1_1464184 antibody, AOR_1_60064 antibody, PGTG_09157 antibody
    Background

    Synonyms: Fructosyl-amino acid oxidase EMBL BAB91123.1 EC=1.5.3

    Background: Fructosyl-amino acid oxidase catalyzed the oxidation of fructosyl-amino acids to produce glucosone, hydrogen peroxide and amino acid. An enzyme that can be used for the determination of glycated proteins in blood.

    Gene Name: faoxC

    UniProt
    Q8RIU8
You are here:
Support