CPA antibody (HRP)

Catalog No. ABIN5596781

Product Details anti-CPA Antibody (HRP)

Target: CPA (Carboxypeptidase A (CPA))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPA antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity (Details): Cross reactivity against Carboxypeptidase A from other tissues and species may occur but have not been specifically determined.

Purification: Carboxypeptidase A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase A [Bovine Pancreas].

Immunogen:

Immunogen: Carboxypeptidase A [Bovine Pancreas]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Carboxypeptidase A is useful in ELISA, western blot, and immunohistochemistry. Optimal titers should be obtained by researchers.

Western Blot Dilution: 1:300 - 1:2,000

ELISA Dilution: 1:3,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CPA

Target: CPA (Carboxypeptidase A (CPA))

Alternative Name: Carboxypeptidase A (CPA Products)

Background:

Synonyms: Carboxypeptidase A1 precursor antibody, CPA1 antibody, Pancreatic carboxypeptidase A1 antibody, Procarboxypeptidase A1 pancreatic antibody

Background: Carboxypeptidase A (CPA-1) is produced in the pancreas and is crucial to many processes in the human body to include digestion, post-translational modification of proteins, blood clotting, and reproduction. There are two proposed mechanisms for the catalytic function of carboxypeptidase A. The first is a nucleophilic pathway involving a covalent acyl enzyme intermediate containing active site base Glu-270. The second proposed mechanism is a promoted water pathway. This mechanism involves attack of a water molecule at the scissile peptide linkage of the substrate.

Gene Name: CPA1

Gene ID: 286762

UniProt: P00730