Acetylcholinesterase antibody (HRP)

Catalog No. ABIN5596812

Product Details anti-Acetylcholinesterase Antibody (HRP)

Target: Acetylcholinesterase (AChE)

Reactivity: Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This Acetylcholinesterase antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Acetylcholinesterase from other sources is unknown.

Purification: Anti-Acetylcholinesterase (Bovine Erthryocytes) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Acetylcholinesterase [Bovine Erythrocytes].

Immunogen:

Immunogen: Acetylcholinesterase [Bovine Erythrocytes]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Acetylcholinesterase (Bovine Erthryocytes) antibody has been assayed against 1.0 μg of Acetylcholinesterase [Bovine Erythrocytes] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Alternative Name: Acetylcholinesterase (AChE Products)

Synonyms: ACEE antibody, ARACHE antibody, N-ACHE antibody, YT antibody, Ache antibody, GB14873 antibody, zgc:92550 antibody, ACE antibody, Dsim\\GD20515 antibody, GD20515 antibody, dsim_GLEANR_4292 antibody, ache antibody, mE1a antibody, mE1b antibody, mE1c antibody, mE1c-long antibody, mE1d antibody, mE1d' antibody, mE1e antibody, arache antibody, n-ache antibody, acetylcholinesterase (Cartwright blood group) antibody, acetylcholinesterase 2 antibody, acetylcholinesterase antibody, Acetylcholine esterase antibody, acetylcholinesterase (Cartwright blood group) L homeolog antibody, collagen type I alpha 2 chain antibody, ACHE antibody, AChE-2 antibody, Ache antibody, ache antibody, Dsim\Ace antibody, ache.L antibody, COL1A2 antibody, ACE-1 antibody

Background:

Synonyms: Acetylcholinesterase, AChE, HRP, Horseradish peroxidase

Background: Acetylcholinesterase, or AChE, is an important protease that terminates the transmission of the neurotransmitter, acetylcholine, by hydrolysis. Hydrolysis by AChE on ACh prevents excessive neural firing between adjacent nerves. Horse Radish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Acetylcholinesterase (Bovine Erthryocytes) antibody is ideal for investigators in Signal Transduction, Cell Signaling, Cardiovascular , and Immunology research.

Gene Name: ACHE

Gene ID: 540446

UniProt: P23795

Pathways: Skeletal Muscle Fiber Development