L1/ORF2 (Internal Region) antibody
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- Target
- L1/ORF2
- Binding Specificity
- Internal Region
- Reactivity
- Human, Mouse
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Host
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Chicken
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- ELISA
- Cross-Reactivity (Details)
- Reactivity occurs against mouse L1/ORF2 protein and is useful in determining its presence in various assays. A BLAST analysis shows reactivity with L1/ORF2 proteins from mouse sources based on 100% homology with the immunizing sequences. Cross reactivity against L1/ORF2 from other sources is unknown.
- Purification
- Anti-L1/ORF2 Antibody is directed against three regions within the endonuclease domain of L1 ORF2 protein. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.
- Immunogen
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Immunogen: This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with three synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.
Immunogen Type: Peptide
- Isotype
- IgG
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anti-L1/ORF2 (Internal Region) antibody
Reactivity: Human, Chimpanzee WB, ELISA Host: Chicken Polyclonal unconjugated
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- Application Notes
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Application Note: L1/ORF2 Antibody has been tested for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 149.5 kDa in size corresponding to L1 protein by western blotting in the appropriate cell lysate or extract.
ELISA Dilution: 1:20,000-1:100,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.21 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- L1/ORF2
- Background
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Synonyms: L1 (LINE-1), Long INterspersed Element , retrotransposon ORF2.
Background: L1/ORF2 is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The L1 (LINE-1), or Long INterspersed Element , retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retro-transposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons.
Gene Name: ORF2
- UniProt
- P11369
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