Phosphoserine antibody (pSer) (Biotin)

Catalog No. ABIN5596874

Product Details anti-Phosphoserine Antibody (Biotin)

Target: Phosphoserine

Binding Specificity: pSer

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phosphoserine antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Cross-Reactivity (Details): Reactivity is specific for phosphoserine and minimal cross reactivity is observed against phosphotyrosine or phosphothreonine.

Purification: Anti-PhosphoSerine (pS) (RABBIT) Antibody Biotin Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated serine peptide coupled to agarose followed by conjugation to biotin and subsequent purification. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin.

Immunogen:

Immunogen: Anti-PhosphoSerine (RABBIT) Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with phosphoserine conjugated KLH.

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-PhosphoSerine Antibody Biotin Conjugated reacts specifically with phosphoserine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphothreonine residues. Anti-PhosphoSerine Antibody Biotin Conjugated reacts with free phosphoserine, phospho-serine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphoserine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry. Specific conditions should be optimized by user.

Western Blot Dilution: 1:500

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:5,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 40 % (v/v) Glycerol

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Phosphoserine

Target: Phosphoserine

Abstract: Phosphoserine Products

Target Type: Amino Acid

Background:

Synonyms: phospho-Ser antibody, pS antibody, pSER antibody, Phosphoserine [pS] reactive antibody, Phosphoserine [pS] polyclonal antibody, Phosphoserine antibody produced in rabbit

Background: PhosphoSerine Antibody Biotin Conjugated is suitable for use in ELISA and western blotting assays. Phosphoserine is an ester of serine and phosphoric acid. Phosphoserine is a component of many proteins as the result of posttranslational modifications. The phosphorylation of the alcohol functional group in serine to produce phosphoserine is catalyzed by various types of kinases. Phosphoserine antibody will detect proteins phosphorylated on serine residues. Anti-PhosphoSerine antibody is ideal for investigators involved in Cancer, Neurocience and Cell Signaling Research.