ALT antibody (HRP)

Catalog No. ABIN5596903

Product Details anti-ALT Antibody (HRP)

Target: ALT (Alanine Aminotransferase (ALT))

Reactivity: Pig

Host: Sheep

Clonality: Polyclonal

Conjugate: This ALT antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Alanine Transaminase (ALT) from other sources is unknown.

Purification: Alanine Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Alanine Transaminase (ALT) [Pig Heart].

Immunogen:

Immunogen: Alanine Transaminase (ALT) [Pig Heart]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Alanine Transaminase has been assayed against 1.0 μg of Alanine Transaminase (ALT) [Pig Heart] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:40,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ALT

Target: ALT (Alanine Aminotransferase (ALT))

Alternative Name: Alanine Transaminase (ALT Products)

Synonyms: AAT1 antibody, ALT1 antibody, GPT1 antibody, GPT antibody, Gpt1 antibody, An11g02620 antibody, AO090003000164 antibody, 233.t00009 antibody, 24.t00016 antibody, 1300007J06Rik antibody, 2310022B03Rik antibody, ALT antibody, Gpt-1 antibody, AU021132 antibody, Dpagt2 antibody, Gnpta antibody, gpt antibody, ALANINE AMINOTRANSFERAS antibody, AtAlaAT1 antibody, AtAlaATc antibody, T13M22.3 antibody, T13M22_3 antibody, alanine aminotransferas antibody, glutamic--pyruvic transaminase antibody, glutamic-pyruvate transaminase (alanine aminotransferase) antibody, alanine aminotransferase antibody, alanine aminotransferase 1 antibody, glutamic pyruvic transaminase, soluble antibody, dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) antibody, alanine aminotransferase 2-like antibody, Alanine aminotransferase 1, mitochondrial antibody, GPT antibody, Gpt antibody, Tb927.1.3950 antibody, ANI_1_368094 antibody, AOR_1_284154 antibody, ALAT_1 antibody, ALAAT1 antibody, EHI_159710 antibody, EHI_096750 antibody, LOC5572541 antibody, ALAT antibody, AAT2 antibody, Dpagt1 antibody, LOC100537633 antibody, AlaAT1 antibody

Background:

Synonyms: AAT1 antibody, Alanine aminotransferase 1 antibody, alanine aminotransferase antibody, ALT 1 antibody, ALT1 antibody, Glutamic alanine transaminase 1 antibody

Background: Alanine Transaminase is a transaminase enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Alanine Transaminase is found in plasma and in various bodily tissues, but is most commonly associated with the liver. It participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.

Gene Name: ALT

Gene ID: 2875

UniProt: P13191