ARG antibody (HRP)

Catalog No. ABIN5596906

Product Details anti-ARG Antibody (HRP)

Target: ARG (Arginase (ARG))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARG antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Arginase from other tissues and species may occur but have not been specifically determined.

Purification: Arginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Arginase [Bovine Liver].

Immunogen:

Immunogen: Arginase [Bovine Liver]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Arginase has been assayed against 1.0 μg of Arginase [Bovine Liver] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:40,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ARG

Target: ARG (Arginase (ARG))

Alternative Name: Arginase (ARG Products)

Synonyms: CG18104 antibody, Dmel\\CG18104 antibody, EG:171D11.4 antibody, EG:65F1.3 antibody, SI:zC146F4.4 (novel protein with NUDIX domain) antibody, si:ch211-146f4.3 antibody, Tb08.26N11.490 antibody, rocF antibody, NV10213 antibody, argi1 antibody, arginase antibody, arginase 1 L homeolog antibody, arginase 1 antibody, Arginase-1 antibody, arg antibody, arg1.L antibody, arg1 antibody, ARGAH1 antibody, BP0538 antibody, rocF antibody, SAS2066 antibody, RR_RS05730 antibody, CND03500 antibody, CNG00550 antibody, Tb927.8.2020 antibody, PGTG_16455 antibody, LOC100123155 antibody, argi1 antibody

Background:

Synonyms: A I antibody, Al antibody, ARG 1 antibody, ARG1 antibody, Arginase 1 antibody, Arginase liver antibody, Arginase type I antibody, Arginase1 antibody, Liver type arginase antibody, Type I arginase antibody

Background: Arginase is a manganese-containing enzyme belonging to the ureohydrolase family of enzymes. Arginase catalyzes the fifth and final step in the urea cycle, a series of biochemical reactions in mammals during which the body disposes of harmful ammonia. Specifically, arginase converts L-arginine into L-ornithine and urea.

Gene Name: ARG1

Gene ID: 513608

UniProt: Q2KJ64